To alleviate the psychological stress using accurate mindfulness practices, this research team will proudly present one novel integrated platform of Mindfulness with Intelligent Neuro-feedback and Disciplinary Virtual Reality (MINDVR) to assist mindfulness practitioners. The new platform originates from brain mechanisms by extracting temporal features based on spatial constraints and applying neuro-feedback with virtual reality (VR). We plan to recruit forty experienced mindfulness practitioners and sixty novice practitioners with an 8-week mindfulness intervention for training and testing in the current proposal. Specifically, the project will be initiated by differentiating mindfulness practices into four phases: mindfulness of breath, body scan, mindfulness of emotions, and compassion (subproject 1). Participants will participate in different stages of mindfulness practices and corresponding cognitive tasks (subproject 5), meanwhile performing the simultaneous electroencephalography (EEG)-functional magnetic resonance imaging (fMRI) recordings to extract the brain locations and temporal features, correspondingly (subproject 2). The targeting EEG temporal biomarkers will be extracted using deep learning for improving the quality of mindfulness practices (subproject 3). After synchronizing the EEG signals and the physiological signals (e.g., heartbeat pulsation), we will integrate biomarkers into a VR environment for practitioners to self-regulate their mindful way of living (subproject 4). The research team will be collaborated with two highly recognized international scholars and be supported by well-known industrial partners toward benefiting the mental health of middle-aged and senior populations in integrated teamwork.