臨場觀測並探討音樂對於大腸桿菌之影響

In-situ monitoring the effect of acoustic vibration in the form of music on the motility of Escherichia coli

Sustainable Development Goals

Abstract/Objectives

To study the effect of music on bacteria and its potential mechanism, a new analytical approach combining optical microscope with software was carried out. Classical music (Flight of the Bumblebee) with different frequencies and tempos was transmitted through bone conduction apparatus to stimulate Escherichia coli MG1655. The increment of E. coli motility affected by Flight of the Bumblebee was quantified by indirect motility assay and in-situ monitoring. Results showed that E.coli MG1655 perform higher motility under higher frequency and faster tempo. We suggest this new analytical approach could be applied for investigating the motility of E. coli. Moreover, it could be a potential mechanism to modulate biological cells for future digital therapeutic and medical device applications. (source: https://doi.org/10.1016/j.apacoust.2020.107620)

Results/Contributions

This study found that the use of solid vibrations to transmit sound waves was more effective in promoting the motility of E. coli. By further adjusting the frequency and rhythm of Flight of the Bumble Bee, we found that the higher frequency and faster rhythm was most effective in promoting the swimming ability of E. coli. It was found that High fast music could even increase the average swimming speed of bacteria by 139%.

In terms of growth, we found that Mid slow, which has a lower frequency and slower rhythm, can promote the growth of E. coli most effectively.

In comparing the effects of music on the swimming ability and growth of E. coli, we found that the music elements needed to promote the movement and growth of E. coli were opposite, with high frequency and fast rhythm music promoting the movement of E. coli and low frequency and slow rhythm music promoting the growth of E. coli. Thus, it is inferred that music of different frequencies and rhythms can stimulate different mechanical force receptors in the membrane of E. coli, resulting in different changes in the movement and growth of the bacteria.

From this study, we found that music of different frequencies and rhythms can affect the motility and growth of E. coli, and this mechanism can be further investigated for future applications in sewage prevention and treatment, antifouling of medical devices, auxiliary bioengineering, and even promoting the productivity of plant and animal related industries.

(source: https://doi.org/10.1016/j.apacoust.2020.107620

https://hdl.handle.net/11296/sr7vkq)

Keywords

SoundFrequencyTempoMotilityIn-situ monitoringEscherichia coli MG1655

References

1. https://doi.org/10.1016/j.apacoust.2020.107620

1. Ku, Hsin-Ning, et al. "In-situ monitoring the effect of acoustic vibration in the form of music on the motility of Escherichia coli." Applied Acoustics 172 (2021): 107620. (https://doi.org/10.1016/j.apacoust.2020.107620) 2. 臨場觀測並探討音樂對於大腸桿菌之影響 (https://hdl.handle.net/11296/sr7vkq)