In this project, we propose the use of alkyne derivatives to form stable gold-alkyne covalent bonds with gold nanoparticles, thereby functionalizing the gold nanoparticles and applying them in lateral flow chromatography to develop a rapid and simple real-time detection tool for monitoring highly reactive molecules, organic small molecules, and enzyme activities. Our preliminary results have demonstrated that alkyne derivatives can effectively functionalize gold nanoparticles. In this project, we modified biotin with an alkyne group and then directly attached it to the surface of gold nanoparticles. These alkyne-modified gold nanoparticle-biotin conjugates will be applied to a novel signaling concept we developed in 2018 - a molecule sensor with tunable affinity. We expect that this new method of coupling gold nanoparticles will make a significant contribution to the field of rapid and simple chemical sensing. We also believe that the innovative application of combining alkyne-modified gold nanoparticle-biotin conjugates with lateral flow chromatography techniques will provide greater convenience for non-professional users who require rapid and straightforward real-time testing.