while female mice were often understudied in behavioral research due to the variation caused by the estrous cycle, whether females really showed larger variation than males remains an uncertain assumption. To validate this hypothesis, in this study, we applied four different inbred strains of female mice, C57BL/6, BALB/c, C3H, and DBA/2 to examine multiple non-sexual behaviors, including anxiety-like, depression-like behaviors and the same-sex interaction, between females in estrus and diestrus stages to test if the estrous cycle has an impact on any of these behaviors. The variation of these behaviors between C57BL/6 female and male mice was also compared to ask if the female mouse behavioral variation was greater than males. We are hoping that this study would help us clarify the exact influence of the estrous cycle on female mouse behaviors. The information could be an important guidance for future experimental design in behavioral neuroscience.