Our research focuses on developing optical and histological tools for 3-D characterization of tissue microstructure, vasculature, and innervation. We prepare transparent specimens (e.g., mouse and human intestine, pancreas, and liver) with optical clearing to reveal the fluorescently labeled tissue architectures in space. We integrate modern 3-D histology (fluorescence and transmitted light signals) and classic microtome-based histology (H&E and IHC signals) for multiplex human tissue analysis in health and disease. Since 2018, we have successfully transformed our 3-D imaging platform from mouse to human pancreas analysis (note: rodent and human pancreases are markedly different in lobular structure, exocrine-endocrine association, and islet physiology). Our Pancreas Team TW (https://3d-histology.com/human-pancreas-new.html) has integrated the fields of surgery (Dr. YW Tien, NTUH), pathology (Dr. YM Jeng, NTUH), and modern 3-D histology to investigate the human pancreas in health and disease. We are currently performing a team-based human pancreas analysis in Taiwan for organ-wide investigation of exocrine-endocrine relationship in early pancreas remodeling. In particular, the 3-D image data has been used to identify the pancreatic tissue networks, such as the neuro-insular network. The classic microtome-based 2-D histology provides the clinical information of the disease area. The integration of modern 3-D and classic 2-D histology allows us to characterize the local human islet remodeling with high definition.