Sustainable Development Goals
Abstract/Objectives
This study introduces a novel biotin and streptavidin-based affinity switch that enables a tunable "signal on" small molecule lateral flow assay (LFA) through spatial disruption. The focus is on a simple, modular method for the specific detection of methotrexate using adjustable affinity lateral flow chromatography (ASLFA). The researchers suggest that this innovative adjustable affinity technique could be beneficial for future drug development and clinical diagnostics, addressing existing challenges in small molecule detection.
Results/Contributions
In this study, we developed a biotin and streptavidin-based affinity switch that is adjustable through spatial disruption, creating a "signal on" small molecule lateral flow assay (LFA) with a switchable effect. Specifically, we present the results of a simple and modular adjustable affinity lateral flow chromatography (ASLFA) method for the specific detection of methotrexate. We believe that the above adjustable affinity approach can also be applied in the future to new drug development and clinical diagnostics, overcoming the limitations of small molecule detection.
Keywords
Gold NanoparticlesLateral Flow Chromatography BioconjugationSmall Molecules